Difference between revisions of "5S ribosomaalne RNA"

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Korrastasin skripti abil viiteid
m (Transkriptsioon (geneetika))
m (Korrastasin skripti abil viiteid)
 
[[Pilt:PDB 1c2x EBI.png|thumb|[[Transmissioonelektronmikroskoop|Krüoelektronmikroskoopia]] põhjal koostatud kujutis ''[[Escherichia coli]]'' 5S rRNA molekulist.<ref name="pmid10756104">{{cite journal |author=Mueller F, Sommer I, Baranov P, Matadeen R, Stoldt M, Wöhnert J, Görlach M, van Heel M, Brimacombe R |title=The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution. |journal=J Mol Biol |volume=298 |issue=1 |pages=35–59 |year=2000 |pmid=10756104 |doi=10.1006/jmbi.2000.3635}}</ref>]]
[[Pilt:PDB 2gya EBI.jpg|thumb|[[Ribosoom]].<ref name="pmid16713583">{{cite journal |author=Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J |title=Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements. |journal=Mol Cell |volume=22 |issue=4 |pages=533–43 |year=2006 |pmid=16713583 |doi=10.1016/j.molcel.2006.05.003}}</ref>]]
 
'''5S ribosomaalne RNA''' ('''5[[Svedberg|S]] rRNA''') on nii [[prokarüoot]]ide ([[50S]]) kui ka [[eukarüoot]]ide ([[60S]]) suurte ribosomaalsete alaühikute komponent. 5[[Svedberg|S]] rRNA on ligikaudu 120 [[nukleotiid]]i pikk, mis on küllaltki lühike võrreldes teiste [[ribosomaalne RNA|ribosomaalsete RNA]]-dega. 5S rRNA-d ei leidu seente [[mitokonder|mitokondriaalsetes]] ribosoomides.<ref name="pmid11752286">{{cite journal |author=Szymanski M, Barciszewska MZ, Erdmann VA, Barciszewski J |title=5S Ribosomal RNA Database |journal=Nucleic Acids Res. |volume=30 |issue=1 |pages=176–8 |date=jaanuar 2002 |pmid=11752286 |pmc=99124 |doi= 10.1093/nar/30.1.176|url=}}</ref>
 
Eukarüootset 5S rRNA-d sünteesib [[RNA polümeraas III]], samas kui enamik teisi eukaroüootseid rRNA-sid toodetakse 45S prekursorilt, mida transkribeerib [[RNA polümeraas I]]. On näidatud, et ''[[Xenopus]]''<nowiki>'</nowiki>e [[ootsüüt]]idel üheksa [[tsink-sõrm]]elise [[transkriptsiooni faktor]]i TFIIIA sõrmed 4–7 võivad seonduda 5S RNA tsentraalse piirkonnaga.<ref name="Searles_2000>{{cite" journal | last = Searles | first = MA |author2=Lu D|author3=Klug A | year = 2000 | title = The role of the central zinc fingers of transcription factor IIIA in binding to 5 S RNA | journal = J Mol Biol | volume = 301 | pages = 47&ndash;60 | pmid = 10926492 | doi = 10.1006/jmbi.2000.3946 | issue = 1}}</ref> Seondumine 5S rRNA ja TFIIIA vahele aitab nii represseerida edasist 5S RNA [[geen]]i [[transkriptsioon (geneetika)|transkriptsioon]] kui ka stabiliseerida 5S RNA transkripti nii kaua kui seda on vaja ribosoomi moodustumisel.<ref name="Pelham_1980>{{cite" journal | last = Pelham | first = HRB |author2= Brown DD | year = 1980 | title = A specific transcription factor that can bind either the 5S RNA gene or 5S RNA | journal = Proc. Natl. Acad. Sci. USA | volume = 77 | pages = 4170&ndash;4174 | pmid = 7001457 | doi = 10.1073/pnas.77.7.4170 | issue = 7 | pmc = 349792}}</ref>
 
==Interaktsioonid valkudega==
5S rRNA seondub prokarüootidel ribosomaalsete valkudega L5, L18 ja L25. Eukarüootidel seondub 5S rRNA ainult [[Ribosomaalne valk L5|ribosomaalse valgu L5-ga]].<ref name="pmid11258942">{{cite journal |author=Moore PB |title=The ribosome at atomic resolution |journal=Biochemistry |volume=40 |issue=11 |pages=3243–50 |date=märts 2001 |pmid=11258942 |doi= |url=}}</ref>
 
==Avastamine==
5S ribosomaalse RNA esmaleidjate sekka kuulub [[Artur Lind]] [[Tartu Ülikool|Tartu Riiklikust Ülikoolist]].<ref>[[Margus Maidla]]: [http://www.sirp.ee/s1-artiklid/c21-teadus/ruum-kus-sundis-eesti-molekulaarbioloogia/ name="Ruum, kus sündis Eesti molekulaarbioloogiaAdOnI"] Sirp, 14. oktoober 2016</ref>
 
==Viited==
{{viited}}|allikad=
<ref name="pmid10756104">{{cite journal |author=Mueller F, Sommer I, Baranov P, Matadeen R, Stoldt M, Wöhnert J, Görlach M, van Heel M, Brimacombe R |title=The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution. |journal=J Mol Biol |volume=298 |issue=1 |pages=35–59 |year=2000 |pmid=10756104 |doi=10.1006/jmbi.2000.3635}}</ref>
<ref name="pmid16713583">{{cite journal |author=Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J |title=Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements. |journal=Mol Cell |volume=22 |issue=4 |pages=533–43 |year=2006 |pmid=16713583 |doi=10.1016/j.molcel.2006.05.003}}</ref>
<ref name="pmid11752286">{{cite journal |author=Szymanski M, Barciszewska MZ, Erdmann VA, Barciszewski J |title=5S Ribosomal RNA Database |journal=Nucleic Acids Res. |volume=30 |issue=1 |pages=176–8 |date=jaanuar 2002 |pmid=11752286 |pmc=99124 |doi= 10.1093/nar/30.1.176|url=}}</ref>
<ref name="Searles_2000">{{cite journal | last = Searles | first = MA |author2=Lu D|author3=Klug A | year = 2000 | title = The role of the central zinc fingers of transcription factor IIIA in binding to 5 S RNA | journal = J Mol Biol | volume = 301 | pages = 47&ndash;60 | pmid = 10926492 | doi = 10.1006/jmbi.2000.3946 | issue = 1}}</ref>
<ref name="Pelham_1980">{{cite journal | last = Pelham | first = HRB |author2= Brown DD | year = 1980 | title = A specific transcription factor that can bind either the 5S RNA gene or 5S RNA | journal = Proc. Natl. Acad. Sci. USA | volume = 77 | pages = 4170&ndash;4174 | pmid = 7001457 | doi = 10.1073/pnas.77.7.4170 | issue = 7 | pmc = 349792}}</ref>
<ref name="pmid11258942">{{cite journal |author=Moore PB |title=The ribosome at atomic resolution |journal=Biochemistry |volume=40 |issue=11 |pages=3243–50 |date=märts 2001 |pmid=11258942 |doi= |url=}}</ref>
<ref name="AdOnI">[[Margus Maidla]]: [http://www.sirp.ee/s1-artiklid/c21-teadus/ruum-kus-sundis-eesti-molekulaarbioloogia/ "Ruum, kus sündis Eesti molekulaarbioloogia"] Sirp, 14. oktoober 2016</ref>
}}
 
[[Kategooria:RNA]]